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five–four μmol/L variety,[156][157] the focus of serotonin in synaptic vesicles was calculated at 270 mM.[182] subsequent vesicular release, the ensuing focus of serotonin in the synaptic cleft, to which serotonin receptors are uncovered, is estimated to generally be about 300 μmol/L. Therefore, although in vitro receptor binding affinities,
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